THE FLAVONOIDS COMPOUNDS OF SEABUCKTHORN
|Number || Chimical Formulation|| Number|| Chimical Formulation|
|5||isorhamnetin-3-Oglucoglucoside||14||isorhamnetin-3-O-gluco-(1® 6) glucoside|
SEABUCKTHORN FLAVONOIDS & THEIR MEDICAL VALUE
Zhao Yuzhen Wu Fuheng(Institute of Information, Shanxi Academy of Agriculture Science,Taiyuan, 030031)
Abstract In this paper, we briefly described the contents, types, extraction methods and medical value of Seabuckthorn flavonoids.
Key words: Seabuckthorn Flavonoids Medical application and development In the medical application of seabuckthorn, flavonoids is one of the compounds that both expertise in and outside the country are focused on. Especially, when its biological activity have been revealed from various aspects by expertise, it caused more and more attention in scientific world.
1. Content of flavonoid compounds in seabuckthorn
Flavonoid compounds existed in all part of seabuckthorn— root, stem, leaf, flower and
fruit. According to the former Soviet Union researcher, in fresh fruit, the highest contents is 854mg/100g, dried leaf is 3888mg/100g; according to Chinese researcher, the average in fresh fruit is 354mg/100g, leaf is 867mg/100g. Studies also showed that in seabuckthorn from high sea level area, the flavonoids content was higher.
2. Types of flavonoid compounds in seabuckthorn
Using Ultra-violet spectrum, researchers in the former Soviet Union and Czechoslovakia identify the flavonoids in seabuckthorn mainly as isorhamnetin, quercitin,
myricetin and kaempferol and their glucoside compounds. From the attached table, oneHippophae, 1997, vol. 10(1), pp39-41.2 could see that isorhamnetin and quercitin are the main flavonoid glucoside compounds in seabuckthorn. The carbohydrates that form the glucoside are glucose, rhamnose,arabinose, galactose, and mostly are in 3-O-glucoside formation.
3. The extraction and separation of flavonoid compounds in seabuckthorn
Hippophae, 1997, vol. 10(1), pp39-41.3
According to the polar of seabuckthorn flavonoid compounds, it is general extracted
by water : methanol at 1:1 mixture. Before extraction, one could also using chloroform to get ride of lipo-soluble compounds.
Raw extracted compounds could be refined by column chromatography, the normal
selected absorbents are silica gel, nitrocellous or polyacrylamide. The running buffer could also be water and methanol mixture.
In recent years, there were also methods like thin layer chromatography and high
pressure liquid chromatography be used in purifying pure flavonoids, those methods is
good for very small amount samples which is also required for quantity analysis.
For industrial extracting flavonoid compounds, the technology, “ alcohol extraction,water precipitation and alcohol dissolution” method, found by Zhu Rong in China, is one of the best approved method for high quality, high recovery and low cost flavonoid compounds.
4. The pharmaceutical function and its medical application of flavonoid compounds in seabuckthorn Studies by Zhong Fei et al. demonstrated that the seabuckthorn extraction could improve the phagocytosis of the giant phagocytes in mouse, increase the lysozyme content, increase the periphery T lymph cell percentage, and enhance the production of interferon in white blood cell, those function suggest that seabuckthorn extraction could enhance the animal’s non-specific and specific immunization functions.
Demonstrated in various experiments, seabuckthorn total flavonoids could directly
remove the O2- and -OH, and the remove efficient is higher than V-E.
In their studies, former Soviet Union researchers found that seabuckthorn
flavonoids could inhibit the atherosclerosis, decrease the cholesterol level in blood etc..
The effect of seabuckthorn flavonoid on heart and blood system had been extensively
studies by researchers in China, those studies had been carried out in details for about 20 years. From studies by Wang Jieliang, Liu Bingwen in 70’s to studies by Wang Bingwen at 90’s, the results all showed that seabuckthorn flavonoids could significant enhance the Hippophae, 1997, vol. 10(1), pp39-41.4 expansion and contraction function of heart, had significant anti-cardiac muscle blood deficiency and anti abnormal heart rhythms. Wang Bingwen et al. also discussion the mechanism of seabuckthorn flavonoids on increase the contraction ability of cardiac muscle. Calcium is a key compound for the exciting-contraction of cardiac muscle, and seabuckthorn total flavonoids could increase the flow of calcium from outside the cell to inside the cell, and also increase the release of calcium from calcium pools inside cell. The animal experiment by Chai Qiuyang et al. also showed that seabuckthorn flavonoid could significantly increase the anti-hyperlipemia disease caused by high fat forge, and decrease
the lipid in blood and liver, which demonstrated that seabuckthorn had certain means in prevent the coronary heart disease and atherosclerosis. The seabuckthorn flavonoid tablet
produced by Tongliao pharmaceutical company in Inner Mongolia had been clinical tested
in 7 hospitals in Beijing in 303 patients with angina pectoris, and the total effectiveness
rate was 90.88%.
In general, the medical application and development of seabuckthorn flavonoids
compound is one of the necessary and important step for the comprehensive utilization of
seabuckthorn resource. It is a very important project for extracting flavonoids from leaf,
because it do not compete raw material with other seabuckthorn processing projects, as
well as its high content and low cost, and it could also find a processing way for those
male seabuckthorn without any fruit.
1. (In Russian)
2. (In Russian)
3. (In Russian)
4. Chen Tigong et al., Studies on the biochemical composition of Hippophae and its
quality assessment in Gansu province. Hippophae, 1988.
5. Harborne J. B. Comparative biochemistry of the flavonoids, London: Academia Press,
1967.Hippophae, 1997, vol. 10(1), pp39-41.5
6. Raheman J. Et al., The flavonoids, Jirougui acid and carbohydrate in the seabuckthorn
fruit from the west Czechoslovakia. Symposium International Seabuckthorn
Conference, Xian: 1989:89-96.
7. (In Russian)
8. Zhu Rong et al., Studies toward on the extraction technology of flavonoids from
seabuckthorn leaf. National Conference on Seabuckthorn Development, Xian:1993.
9. Zhong Fei et al., Immunological pharmacology studies on the seabuckthorn extraction.
National Conference on Seabuckthorn Development, Xian:1993;268-269.
10. Ju Haisong et al., The removing of free O2- by seabuckthorn total flavonoids. Acta
Chinese Pharmacology, 1990; 6(2):97-101.
11. (In Russian)
12. Liu Binwen et al., Curative effects of seabuckthorn fruit on hyperlipemia and coronary
heart disease. Acta Sichuan Medical School, 1980.
13. Wang Jialiang et al., Curative effects of seabuckthorn total flavonoids on iron deficient
heart disease. Acta Sichuan Medical School, 1982;(1):6.
14. Wang Binwen et al., Effects of seabuckthorn total flavonoids on mouse heart function
and blood dynamics. Symposium International Seabuckthorn Conference, Xian:
15. Wang Binwen et al., Effects of seabuckthorn total flavonoids on human heart function
and blood dynamics. Acta Xian Medical School, 1993; 14(2):130-140.
16. Wang Binwen et al., Effects of Hippophae total flavone upon positive variation force
of transguinea in vitro nipple muscle. Hippophae, 1993; 6(2):23-27.
17. Wang Binwen et al., Effects of Hippophae total flavone upon heart function of
transguinea. Hippophae, 1993; 6(3):28-30.
18. Li Kexu et al., Effects of Hippophae total flavone upon transguine in in vitro cardiac
muscle. Hippophae, 1993; 6(3):28-30.
19. Wang Binwen et al., Effects of Hippophae total flavone upon heart function on in vivo
and in vitro frog heart. Hippophae, 1994; 7(2):42-44.
Hippophae, 1997, vol. 10(1), pp39-41.6
20. Cai Qiuyan et al., Preliminary study on the pharmaceutical effect of seabuckthorn
extraction on heart and blood vessel. Symposium International Seabuckthorn
Conference, Xian: 1989:288-291.
21. Yu Mingyu How to correctly evaluate the anti-cancer function of seabuckthorn and
the direction for further study. National Conference on the Hippophae Medical
Development, Xian: 1993.
SEABUCKTHORN FLAVONOIDDS CAPSULES
The flavonoides of seabuckthorn are maintly extracted from fruit pulps and leaves. Total flavone concentration adds up to more 20%. Appearance is Kelly powder. It was proved by a great deal of experiments that seabuckthorn flavone has the effects to reduce blood fatty., increase shrinkage and diastole, resist cardiac muscle lacking of blood as well as arrhythmia. Therefore, seabuckthorn flavone is ideal in the aspects of medicine and health-care food. Usage guidelines: Sometimes the flavonoid extract is combined with starch; for example, a tablet, a soft and hard flavonoid capsule and taken orally liquid is produced for use in treating cardiovascular disease, regulating blood fatty, anti-aging and increasing immunity. The cardio vascular system diseases were recognized publicly as one of the biggest enemy to threaten human's health and survival. It was showed by the state statistics of 1996, about 15.5 million persons died of CVD. It amounted to 30% of total world death toll died of diseases in that year, and amounted to 45.6% of total death toll in developed countries, 24.5% of total death toll in developing countries. 7.2 million persons died of coronary heart disease that ranked the first place and 4.6 million persons died of cerebral vascular disease that ranked the second place in 1996 among the main death due to diseases. Experts point out that the abnormity of blood and the factors of psychology and society (such as despondence, anxiety) which are incurred from smoking, high fat, high blood pressure, abnormity of blood sugar as well as physical activities, etc. will cause cardiovascular system diseases directly. And flavonoid, VE, VA, VC, as well as amino acid can effectively prevent and reduce the occurrence and development of cardiovascular system diseases. This product contains the natural active substance extracted from seabuckthorn fruits by advanced scientific technology which composed the prescription scientifically according to traditional medical and physiochemical requirements. It can effectively improve the circulation of cardiac and brain vascular that has the function on preventing and curing the cardiovascular system diseases such as coronary heart disease, angina, arrhythmia, cardiac muscles lacking of blood, high blood-fat, etc.. It also can reduce blood viscosity, increase the tenacity of blood vessel and the flow of blood, decrease and eliminate oxy-radical in body, improve immunity and anti-aging. It was approved by China Ministry of Health in 2000, that this product had the health-care function to reduce blood-fat, and it was suitable to the public who are easy to get the cardiovascular diseases. Raw material: Seabuckthorn seed, extracted substance from seabuckthorn berry. Approved file no.: Weishijianzi (2000) No.0426 Executive standard: ZL98103418.7 Ingredients and contents: Each 100g flavone contents as below: Total flavone 600 mg VE 120 mg VC 600 mg Carotenoid 190 mg carotene 7.7 mg Necessary amino acid 260 mg Total amino acid 940 mg Health-care function: Reduce blood-fat Suitable to: person having high blood-fat Administration and Dosage: 4 capsules each time, twice a day, swallow with warm water or take orally Specification: 0.45g/capsule Shelf life: 18 months (see from the bottom of the box) Storage: Keep in a cool, dark and dry place.